FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_48.3520000003924f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_48.3520000003924f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289935
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8630.2976529222067015No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCTGC402.46316E-422.4975418
CTTGGGT350.00299969521.42992623
ATATACC904.2447937E-818.3313313
ATACGTG500.001108315517.9980345
GTATTAG854.6764399E-717.651221
CTATGCA601.8611662E-417.4980899
TCGATTC600.003743332615.00094921
TTAGTCT600.003747632514.9983624
TATAATG1104.1735439E-714.9983622
CTAATGT600.003747632514.9983623
GTATAGA750.001012915414.0033021
GATAAGG650.006375488413.8446417
TAGGTAG650.006375488413.8446415
TATACCT650.006375488413.8446414
GTATATA2500.013.8032531
GTGTAGA902.635979E-413.3364761
GTATAAA850.002599537112.3558541
CCTAATA850.002606276212.3515912
AATTATA900.00399800311.6653932
CTAATAC900.00399800311.6653933
Produced by FastQC (version 0.11.2)