FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_47.3520000003920b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_47.3520000003920b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586452
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9020.15380627911576736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG908.5752254E-716.6647265
GCCTTAG600.003734785115.009771
GGTAGTC1104.1951353E-714.9982527
AGACTGT1054.266316E-614.2840516
GTATTAG1402.9533112E-813.9376431
GGCTATG650.006386060713.8445417
TCACACA902.6434465E-413.336328530
TATTAGG1507.3589035E-812.9995942
CCTAATA2001.2732926E-1012.74962
ACTATAC1651.8566425E-812.7268753
GACATAG1302.7449296E-612.69515830
ACCTAAT1551.1191332E-712.588841
GTAGTCA1101.0423522E-412.27129658
AGTCAGA1601.7093407E-712.1860810
GTGTAAG1006.405513E-412.0078151
AGAGTCA1151.5775848E-411.7377628
CTCCTAT900.003983652711.6742661
GACTGTT900.0040065711.6653087
AGTGTGT900.0040065711.6653088
TTAGGTA1303.840578E-511.5371174
Produced by FastQC (version 0.11.2)