FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_46.352000000391c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_46.352000000391c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399384
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7680.19229613604951626No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCA651.6428876E-518.4597669
GGTAGTC601.8632003E-417.498327
GTATAGA1051.3162207E-817.1476521
TAGGTAG550.002099759916.3620665
TAGTCAG550.002099759916.3620669
AGTGTGC550.002099759916.3620668
AGTCAGA755.6107478E-515.99846510
GTATTAG1052.4582732E-715.7186811
GTAGTCA706.029032E-414.998568
TTAGGAG650.006380489613.8448253
CAACACT650.006380489613.8448254
GGGTTCG650.006380489613.8448257
GTACTAT1106.862527E-613.6401791
GTCAGAT902.6445094E-413.33372411
TTATACA1701.9772415E-913.2340234
CTAATAC1252.5663023E-511.9988483
GGTATAT1151.5693692E-411.74241351
CCAGAAC1151.5748697E-411.7380043
TATACAG2206.220944E-1011.5897965
CATAACT1059.69435E-411.4274744
Produced by FastQC (version 0.11.2)