FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_45.35200000039183.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_45.35200000039183.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271260
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2990.1102263511022635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT559.553139E-519.0887994
GTCTTGA400.006467592418.7652231
TTAAGGC400.00650212718.7479273
ATCACAC500.001106704918.00132829
AAACCCA703.1012176E-517.1409635
CTAATAC703.1012176E-517.1409633
TCACACA550.002095044416.36484330
AACAGTC550.002097627616.3618287
GTATATA1552.910383E-1115.4964411
TACCATT901.574368E-514.9983427
TATGCAA1054.2457195E-614.28413510
ATACTCT750.001015276613.9984516
CATCACA650.00636636813.84717528
CAGCTCA650.0063741513.84462310
AATACTA1106.870774E-613.6348565
CTATGCA1004.251422E-513.4985079
TAATACT1601.1978045E-813.12354854
TAAACCC800.001652110313.12354854
ACCTAAT1056.661996E-512.867581
GTATAAG850.002587588712.3629681
Produced by FastQC (version 0.11.2)