FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_44.35200000039140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_44.35200000039140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430708
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6010.13953769142899597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAA802.4853216E-718.7516691
GTATTAG1056.566552E-1018.5730841
GTGTAGG500.001108029718.0016021
GTCTAAG957.884955E-817.3699681
ATCTTAT550.002097525416.3650931
TCTAGAC550.002099153616.3631943
GTAGGAG653.4310875E-416.1534083
ACAGCGT653.4310875E-416.1534088
TAGCCTG706.027243E-414.9995945
CCTAAGG706.027243E-414.9995943
GCCTCAC706.027243E-414.99959420
ACACTGT706.027243E-414.9995946
GGTAGTC600.003749523514.9995937
GTACTAG750.001015447714.00124551
GGTAGCC750.001016339413.9996218
TGGCGAC650.00637374513.84738630
AATAGAC650.00637374513.84738626
TAGTCAG650.006378649713.8457789
TATTAGG1004.259465E-513.4996342
TAGGTAG902.646527E-413.3329735
Produced by FastQC (version 0.11.2)