FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_43.3520000003910c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_43.3520000003910c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331296
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5370.16209069834830483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAA200.005144309729.99728211
ACCCGTC200.005144309729.9972827
GCCTGTT550.002098666316.3621547
TTTAAAC706.024934E-414.9986423
TCTACAC750.001015956713.9987323
TGATCAC650.00637723213.84495
TAAGGTT650.00637723213.84495
ATAACAC650.00637723213.84493
TCCCTGA800.001641959813.1357091
CTCTAAA800.001641959813.1357091
TAGTCTT800.001653205513.1238125
GTACAAA1354.14434E-612.2321931
ACTTAGA900.003972509411.6761851
TTAAGAG900.00399950511.665613
TATACAA1059.686277E-411.4275365
AGAACAG950.005985386711.0516315
TTAGAAG1100.001429099810.9081033
GTATAGA1253.3818535E-410.8088111
TATAATA1253.4103304E-410.7990232
AAGACTT1150.002069610910.4338385
Produced by FastQC (version 0.11.2)