FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_42.352000000390c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_42.352000000390c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912466
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17420.1909112229935143No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG703.1141164E-517.141045
TAGGTAG750.001018478313.9985165
GCCAGTC650.006389213.8446877
ACTGAGC902.653386E-413.331928
ACCGTCC850.002613626212.3516328
TATACTG3650.012.3274615
ACTATGC2204.7293724E-1112.2714278
GTCTTAG1354.1817875E-612.2282981
CTAAGAC1355.6503646E-511.1099343
ACGGTGT2053.2016032E-810.9744468
AATACAG3350.010.745135
ACCATGC1551.954965E-510.6440338
CTAATAC2402.6484486E-910.6238753
TACACTG1704.6777313E-610.5871125
GTCCTAG1000.00874247910.505221
GTCTAGA1000.00874247910.505221
ACTGTGT1000.00878164510.4988888
ACCGTCG1000.00878164510.4988888
GTATATA8150.010.4960131
GTATAAT4750.010.4262331
Produced by FastQC (version 0.11.2)