FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_41.35200000039084.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_41.35200000039084.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743732
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8650.1163053357930007No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGC500.001109972617.9993958
AGGGTCC550.002101176716.36308715
AATGCGC600.003753102214.999495510
AGACCGC600.003753102214.99949556
CTACCCC600.003753102214.99949554
TCACACA1402.9816874E-813.9290430
CCTATCC650.006384677713.8456883
AAGACCG1004.267886E-513.4995475
TACTATG1958.367351E-1113.0764847
TTATACC1201.6919403E-512.4995794
ACCTAAT1456.8427653E-712.4200551
ACAGTGC850.002611423812.3525278
GCTTATC1354.2037136E-612.2218118
TACTAGA2001.7298589E-912.0004042
TAGACCT1006.4460724E-411.9995985
CTATGCA1501.0377626E-611.9995979
AAGTGCG900.00400581611.6662749
CTAATAC2206.2573235E-1011.590523
GCATTAG1303.819511E-511.5442831
TGTACAG1059.7064767E-411.4281875
Produced by FastQC (version 0.11.2)