FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_40.35200000039041.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_40.35200000039041.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342632
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6830.1993392327628476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCCC452.0361349E-523.3319724
TGCGACG701.4363068E-619.2902230
CCTTGCG701.4403304E-619.2845889
CCCCTTG500.001108496317.9989517
TGCGCTA500.001108496317.99895112
TCAAGAC500.001108496317.9989513
ACCCCTT500.001108496317.9989516
ACCTTGC500.001108496317.9989518
GTGCGAC805.1403313E-616.87894229
AGGTGCG901.573819E-515.00131427
TTGCGCT600.003748217114.99912511
CAGCTAT600.003748217114.9991259
GGTGCGA952.6223368E-514.2117728
CTTGCGC750.001015834413.99918310
CGCTAAA650.006376461613.845345514
ACAGCTA650.006376461613.84534558
CCCTTGC650.006376461613.84534558
TGTATAC1106.8797144E-613.6355683
TAGGTGC1004.249897E-513.50118326
TTATACT1601.2010787E-813.1242334
Produced by FastQC (version 0.11.2)