FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_39.35200000038ff2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_39.35200000038ff2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631837
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9980.15795213005886014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCGG400.006510795618.7482689
CTAGTAC703.113415E-517.1399173
TAGACAG1253.7107384E-1016.7984495
CGTGAAC550.002099308416.36471614
GTATTAG1401.8262654E-915.0104961
CCGGATC600.003751758514.99980225
TAGACTG706.0357526E-414.9986165
GTGCGAC851.6295968E-414.11969629
TATACGG1504.8858055E-913.9987095
CTTATAG902.652834E-413.3310473
GTATAGC800.001646275513.1341841
TGGGCTG954.1860432E-412.6304125
CATGCGG850.002608724212.35375615
TACCCGG850.002610273712.352777522
ACCCGGA850.002610273712.352777523
AGACAGC1101.0423624E-412.2715946
AACACTG1252.5717627E-511.9988935
TAGTACC1754.2233296E-811.9988924
CACGCAG1151.5752758E-411.73990416
TGGCATG900.004006433311.66558911
Produced by FastQC (version 0.11.2)