FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_38.35200000038fcb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_38.35200000038fcb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684375
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9670.14129680365296804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAC859.205563E-615.8799563
TAGTTCG600.003753800414.9988319
TATACCG750.001018367913.9978865
CTAACAC1454.73392E-813.4462453
GTCTATA1201.6897047E-512.5008521
TTCTAAG1804.665708E-912.4981142
TAGGTTG1006.4512755E-411.9981895
AGGAGTC900.004006583211.6657577
TGACCTG900.004008771411.6649065
ACCATGC1059.7083475E-411.4276818
ACACAAG1202.3431673E-411.2491246
ATTATAC2002.2224413E-811.2483023
TCTAAGG1355.6481753E-511.1094333
GTATATA1653.2615517E-610.9098341
TATTATA1851.119437E-610.5389492
TAGACTC1000.00878081210.4984154
CCTTATA1150.002075510310.4332072
CAGGAGT1150.002075510310.4332074
CATGTAC1150.002075510310.4332073
TATAGAA2301.5459591E-810.4332072
Produced by FastQC (version 0.11.2)