FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_37.35200000038f7b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_37.35200000038f7b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987929
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17770.1798712255637804No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGCA350.003006270621.4280649
GACAGCG703.112338E-517.1424527
CCTATCC550.002101693516.363253
ATAGCGC600.003754014814.9996458
CTGCTCG750.001017949513.999679
TGGCGCC650.006386211613.84582616
AGGACAG1006.448834E-411.9997185
ACTGTGC1501.0387848E-611.9997178
GTCCTAT900.0039919811.6722991
TGTTATA2206.2755134E-1011.5906352
ACTGGTC1703.760142E-711.4703188
ACAGCCC950.00599664811.0523718
TATACTG3400.011.0291525
GTCTTAA2053.1754098E-810.9809081
TCTATAG2053.1990567E-810.9753513
TGGGCAG1253.4211675E-410.79974510
ATTATAC3350.010.7460153
GTAGGAC1150.00207471410.4345353
CCTGTAC1304.914083E-410.384373
CTATTAT3401.8189894E-1210.1519571
Produced by FastQC (version 0.11.2)