FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_36.35200000038f38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_36.35200000038f38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497823
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13280.2667614794816632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG752.7757487E-618.0094661
AGACTGT653.4337371E-416.1526016
GTCTTAT859.136602E-615.8907071
ACCTAGA600.003736842215.007891
CTGCTCA809.740886E-514.9988449
GGTATAT1106.853219E-613.6435361
GTATACA1352.8669638E-713.3403461
CTATACT1302.7518508E-612.691334
TCTTATA1354.197871E-612.2212812
CAGCTGG1059.6919894E-411.4288414
TCTATAC1459.30864E-611.3784333
ATTACAC950.00599195111.0517813
TCTACAC1000.00877035710.4991913
TCATATG1000.00877035710.4991912
ATACCAT2002.6741145E-710.4991916
TTAGACT1150.002072524710.4339794
TATAGTA1451.16428375E-410.344032
GTATATA2701.6916601E-910.00525951
CTATTAA1200.00293601910.00525951
CTTAATA1200.00295072729.999232
Produced by FastQC (version 0.11.2)