FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_35.35200000038f01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_35.35200000038f01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883741
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24450.12979491341962615No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCG400.006513934618.74903723
GGACCGT850.002615377512.3516516
ACCGTGC1059.7243267E-411.4273788
TAATACT5400.010.832494
GTATATA8700.010.6939321
ATTAGGG2251.1023076E-810.6658373
CCTTGCG1150.002077610710.4336949
TATCCTG3051.364242E-109.8350385
TATACAG5400.09.7212085
GTATAAG5450.09.6368821
GTCTAAT3750.09.6038411
CTAACCT2352.3667053E-79.5737234
TAGGACT1757.431224E-59.4278374
TAGACAG3051.4206307E-99.34328655
GTATTAG3552.5465852E-119.2994951
GTCCAGT2752.4534529E-89.2764371
GTATTAT5850.09.2344621
ACTGTGT2601.03447746E-79.2298068
TCGTGCA1801.01095655E-49.16570959
TGTACCG1801.01095655E-49.16570955
Produced by FastQC (version 0.11.2)