FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_34.35200000038ecc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_34.35200000038ecc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799622
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13900.17383213568411074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGAC957.922608E-817.3675543
GGTCTAG550.002097206216.3679351
TCTAGGC600.003753840214.99924853
TACAGCG750.001018335713.9984257
AGTGACC650.006388569313.8445968
TAGGACA1201.1216671E-613.7493114
CCTTATA1651.861008E-812.7266372
GGACTGT850.002613268312.351556
GTATATA5200.012.1185671
TAGACTG1006.4512895E-411.9986495
GCCTTAT1303.829789E-511.5414921
TTATACC2103.7307473E-911.42799954
GTATTAG1602.2507866E-611.2529561
GGTTAGG950.005982192211.0555351
GTATTAT3300.010.9119571
TATACTG3051.8189894E-1210.8184555
GGTTAGA1000.00875609210.5027591
TAGGCTG1150.002075503310.4336085
TATTAGG1602.7725035E-510.3119842
TCTACAC1952.1979213E-69.9994993
Produced by FastQC (version 0.11.2)