FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_33.35200000038e89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_33.35200000038e89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293119
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3720.12691091331506998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCAG400.00649791618.7506424
TTAGATA400.00650432118.747444
GTATATA1154.020694E-815.65805151
TAGGGTG650.006369652713.8466275
AGAGCTC650.006369652713.8466278
TATACTG800.001650862913.1254485
CTTACAC800.00165298613.1232093
TTCTATA850.002606927212.3512552
TTATACT850.002606927212.3512554
CTTATAC850.002606927212.3512553
ATACTGA1354.1760723E-612.2226396
TAGTACT1059.684178E-411.42701154
TATACAG1100.001426760910.9094645
ATCTAAT1000.00872658710.50394251
TTAGGGT1000.00875978210.4985674
TCTTACA1000.00875978210.4985672
TCATAGT1000.00875978210.4985672
TAATACA1409.67593E-49.6415414
GAGTTGA1300.00568127739.2342361
TACTGAT1350.00776748968.8891937
Produced by FastQC (version 0.11.2)