FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_32.35200000038e46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_32.35200000038e46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709138
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9740.13734985292002405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC400.006508535718.7497793
TAGAGTG1351.1059456E-915.5542765
GTCCACT600.00375232614.99982412
TAGACTT1002.5723293E-614.9987665
TAGAACC1104.1970998E-714.9987664
TAGGGTG650.006386348513.8450155
CTCGGTC650.006389332513.8440389
GTTCTAG1004.2467036E-513.5065091
ATGGGAC1004.2664615E-513.4998423
GTATAGA1701.9717845E-913.2416741
GCCTCGT800.001655464913.124845519
CCGTTCC800.00165722413.1229948
GTATTAG2001.2732926E-1012.7561461
TGTACTG1302.7566966E-612.6912635
CTAATAC1907.694325E-1012.6314313
TATACTG2401.8189894E-1212.4989725
CGCTCCT850.002609408712.35366626
CTAAGCA850.00261217112.3519254
TTGTCCG850.00261355312.3510539
TAGTACC1951.1623342E-912.3066814
Produced by FastQC (version 0.11.2)