FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_31.35200000038e02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_31.35200000038e02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1639044
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27500.16778073071863844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG4350.013.1084211
CGAGCGT850.002615214612.35159714
CCTAGCG1252.5692301E-512.00272330
GGACTGC1806.278242E-811.665046
GTATTAA5050.011.5885541
GCGCACT1059.7259926E-411.42697911
TACACCG2103.748937E-911.4269795
ATCACAC3050.011.31404129
CAGCGTG1602.2664535E-611.2484339
TAGGTAG2002.2315362E-811.2484335
ACCGTGT1100.001434914310.9075718
CTAGCCT1100.001434914310.9075714
CGCCGCG1253.421632E-410.80047324
TCTATAC4450.010.7850143
TGGACTG2651.09139364E-1010.7532195
GTATTAT7000.010.7183511
ACTATGC4900.010.7127938
CTATGCA3950.010.631439
GTTTAGG1704.65801E-610.5922531
CTAGTAC2853.8198777E-1110.524853
Produced by FastQC (version 0.11.2)