FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_30.35200000038dcd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_30.35200000038dcd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1802796
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29970.16624177111553387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGTC2550.012.9406157
GTATTAG5150.012.8206661
TAGGACT1456.906521E-712.4132544
TCGCCCG850.002613562912.35274824
CTAGGAC1601.7153616E-712.1869713
CTGATCG1252.5774205E-511.99914559
GTATTAA6300.011.6713391
GTTCGCG900.004010726711.6658369
GCCTAAT1202.3368164E-411.2545061
TAGGTAG2555.4569682E-1111.1759855
ACGGTGT3150.010.9516018
GTATAGA5850.010.7735431
ACTGCCC1704.684014E-610.58748158
GTCTTAA2301.5405021E-810.4389621
GTACCGT1756.618704E-610.2852676
TATACTG8000.010.1245595
CCTAGAC1200.00295631389.9995653
TAGCCTG1653.8972772E-59.9995655
TACACCG1809.256841E-69.9995655
GCGCACA1200.00295699329.99928911
Produced by FastQC (version 0.11.2)