FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_29.35200000038d8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_29.35200000038d8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711473
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23660.13824348967234656No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTCG750.001019000313.9987329
CCGCGGA650.00639156313.8449019
CCAGGAC1302.762521E-612.6915293
TACGGCT1302.763425E-612.6911594
ATAGGAC2204.9112714E-1112.2719743
CGGGTCC900.004004166511.66833825
ACTGTGC1859.174437E-811.3503248
TAATACT9250.011.3503234
GTCTTAG1901.3166755E-711.0577691
TAGGACT1951.8990067E-710.7682544
TAGGACA2104.573849E-810.7133154
CGGACGG1000.00878592110.49904913
GTATAGA5750.010.4396321
TAGACAG3051.2732926E-1110.3269335
GTATATG7050.010.2175131
GTATTAG5000.010.2047411
CTAGGAC1653.89773E-59.9993873
TAGTACT4500.09.9990954
ACTCCTC2454.1358362E-89.7950328
CTAATAC5250.09.713693
Produced by FastQC (version 0.11.2)