FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_28.35200000038d47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_28.35200000038d47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456008
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23180.15920242196471446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG2650.014.1496955
TAGTCAG2200.013.6346939
GTATTAG4900.013.1670791
GGTAGTC2600.012.691197
CTATGCA3200.012.1860089
CAGCGTG900.004011631511.6652379
CTATACA3950.011.3917934
CCGTTCC1355.6566725E-511.1097519
ACTATGC4050.011.109758
TCACACA2751.8189894E-1110.91037830
GTGTAGA2802.5465852E-1110.7173891
CTAATAC4350.010.6894473
TTAGGTA4350.010.689084
TACAGCG1704.6829573E-610.5873017
CGGGATG1000.00878023210.49979614
TCTATAC3800.010.2629593
TACTATG5700.010.2622537
GTATAGA3900.010.0028971
AGTCAGA3001.0004442E-109.99877510
AGAGTCC1809.261619E-69.9987758
Produced by FastQC (version 0.11.2)