FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_27.35200000038ce8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_27.35200000038ce8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824549
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27580.15116064298629414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCG550.00210275516.36308514
CCCGGAT1456.901282E-712.41405424
ACCCGGA1501.0398271E-612.00025223
GTATTAG5400.011.11621951
ATTAGGC2207.759809E-910.9084243
ACTATGC5250.010.8564788
CTTACAC2351.877197E-910.85043
TAGGACT1408.1865815E-510.7133374
GACCGTG1150.002076810210.4341447
ACAGCGC1150.002076810210.4341448
CCGGATC1756.61159E-610.28621325
GTATATA17000.010.1517231
ATACGGT5500.010.0902916
GTATAGA6000.010.0045971
TACCCGG1809.249949E-610.00020922
GGACTGG1653.898106E-59.9993896
CTAATAC5400.09.9993893
ACGGTGT3900.09.9993888
TATACGG5250.09.9991145
TAATCCG1200.00295745059.9991135
Produced by FastQC (version 0.11.2)