FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_26.35200000038ca5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_26.35200000038ca5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241160
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21470.1729833381675207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGTC1800.022.497437
TAGGTAG2350.017.2320755
TAGTCAG2200.016.3617699
GTATTAG4550.016.161121
CTAATAC4500.014.6655843
ATCACAC3000.014.00291429
ACCGTGT750.00101890513.9984028
GTCCTAC650.006367416613.8523861
GCCGCGA650.00638955113.84513216
CTATGCA3700.013.782219
AGTCAGA2850.013.68264910
CGATCAC902.649349E-413.33503524
TTAGGTA3150.013.3318114
ACCTAAT3950.012.9172041
TCTATAC3600.012.9157133
GACATAG2950.012.71451230
TATTAGG3950.012.5307192
CCTAATA4350.012.4128762
CGATTAG850.002614701412.35153214
GTGTGTC850.002614701412.3515329
Produced by FastQC (version 0.11.2)