FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_25.35200000038c61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_25.35200000038c61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591735
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14840.25078793716782005No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG350.003007691821.424599
TAGGTAG951.72804E-1020.52255
GGTAGTC802.4964356E-718.7465157
GCTGGGT1001.4181933E-716.50111617
GTATTAG1750.016.2923241
GACTGAT653.4379575E-416.1508437
CAGGACT859.19702E-615.8807434
CGTGAGC951.5075784E-615.78787212
GTGAGCT1052.474826E-715.71269213
CGTCGTG1002.5643258E-615.00228230
CTGGGTT1104.1865678E-715.00101618
CTAATAC1401.8462742E-914.9984793
ACTGTGC809.75488E-514.9972118
TAGTCAG901.5817557E-514.99721059
GGAACGT1251.1321208E-714.3973238
CCTAGCG650.006374791713.84826130
CGATCAC650.00637836313.8470924
ATTAGGT1301.8260107E-713.8447493
ACGTGAG1106.9030593E-613.63498111
TATTAGG1803.274181E-1013.3319812
Produced by FastQC (version 0.11.2)