FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_24.35200000038c11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_24.35200000038c11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643145
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35010.5443562493683384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG751.2631608E-720.0002616
TCGGTCG559.5871845E-519.0867029
GCGCTAC651.6438864E-518.46177917
CGGGGGC904.2628017E-818.3335717
ATATGAC1750.017.9974333
GCTCCCG601.8638793E-417.50022716
GGACCTG601.8672076E-417.4961455
CGCGCTA703.1086307E-517.1430816
CCTGGTG1253.7107384E-1016.79638
GGGACGG908.5877036E-716.6629949
CAAGAGT1203.9090082E-916.246424
GCTACAC653.433331E-416.15405719
CTATGCA1401.07320375E-1016.0678889
TGTAGAC951.5087226E-615.7872233
GTCGGGG951.5099467E-615.785995515
ATCACAC1206.834307E-815.003695529
CGCTACA809.740445E-515.000195518
CCTAGAC706.038264E-414.9978612
TTAGGCA600.003757028414.99669554
GTCGCTT600.003757028414.996695511
Produced by FastQC (version 0.11.2)