FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_23.35200000038bdc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_23.35200000038bdc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619387
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23920.14771021380312427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG4900.014.3978911
CTATGCA4200.013.2129799
TAGGTAG2850.013.1565955
CGATCAC1201.693834E-512.50030924
CCTAGCG1101.0419628E-412.27454730
TAGACTG1601.7161801E-712.1862965
TCGGCGC1252.5753241E-511.99992721
CGGCGCC1151.5792537E-411.73905822
ACGGTGT2701.8189894E-1211.6655148
GGTAGTC2206.2937033E-1011.5897657
CCTAATA5150.011.3588032
GTGTAGG1202.329958E-411.2579261
CTAATAC5600.011.2488893
TATTAGG5100.011.1760572
ACTATGC5400.011.1100148
ATCACAC3600.010.8349429
CCCGACC1408.175864E-510.7145524
GTCTATA3800.010.6654041
GGCGCCA1551.9553865E-510.64509523
GGGTTCG1704.684607E-610.5871897
Produced by FastQC (version 0.11.2)