FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_22.35200000038b99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_22.35200000038b99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765163
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13410.17525677535374815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTC805.170801E-616.8742288
TAGGTAG1451.0913936E-1116.5498855
GTATTAG2650.015.2913141
ACCATGC600.003753535714.9993138
CTAATAC2400.014.3734033
GGACGTA952.6340022E-514.20987514
CGTAATC851.6329523E-414.11700217
GTGTACG650.00636057213.8536691
CTATGCA1507.3718184E-812.9994059
CTGTGCA954.186193E-412.63100059
TTAGGTA1907.712515E-1012.6301764
TGTTAGG850.0026117812.3523762
TAATACT3700.012.160814
CAAGTCC1006.443563E-412.00023620
TAAACTG1252.5739255E-511.9986675
ACTATGC1901.04028E-811.8415628
TTAGGGT900.00400831711.6653714
ATACTGT2751.8189894E-1211.4540216
ATCACAC1202.3417344E-411.2502229
GTATAAC1755.424372E-711.1489051
Produced by FastQC (version 0.11.2)