FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_21.35200000038b56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_21.35200000038b56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128996
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4880.37830630407144406No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATG200.00513896429.9899211
CTACACT200.00513896429.989924
GACGCGC200.00513896429.989928
ACGCGCA200.00513896429.989929
ACCTGCG455.4428173E-419.9932797
CCTAAGG400.006477975818.7509693
AATACTA400.00649250918.74375
TAATACT550.002092753816.3581394
CTCTTGA600.00371898715.00659751
TACTACT600.003738252414.994964
Produced by FastQC (version 0.11.2)