FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_20.35200000038b12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_20.35200000038b12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347284
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8390.24158901648218747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCA608.230538E-619.99817710
GGTAGTC608.230538E-619.9981777
TAGGTAG901.9517756E-919.9981755
AGTGTGC455.4593716E-419.9981758
TACACAG559.561541E-519.0891695
ATACACG400.00649957918.7509917
GTATTAG1056.530172E-1018.5777591
GCTTAAC651.6357131E-518.4678331
ACCTAGC651.6397784E-518.46251329
GACGATC651.6397784E-518.46251322
CGATCAC651.6397784E-518.46251324
GACTAGG651.6418142E-518.4598548
ACAGTCC500.001108781717.998368
CACCTAG703.1009153E-517.14376328
AGACTAG805.1566603E-616.873467
TAGAGAC908.54825E-716.6651464
GTCCCTA550.002092897416.3692151
CCGTTAT550.002092897416.3692151
GGACCCT550.0020989516.3621446
GTATAGA1203.8598955E-916.2555391
Produced by FastQC (version 0.11.2)