FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_19.35200000038add.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_19.35200000038add.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1717682
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28140.16382543450999662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCGT1750.017.99989524
TCCCGCC1800.016.6665722
CCCGCCG1750.016.28514523
GGTAGTC2800.015.53517157
CTCCCGC1850.015.40531521
GAGAGTC2450.015.3055897
TAGGTAG3450.015.216865
GTATTAG4650.014.5223861
TAGTCAG3050.014.2617979
ACTCCCG2000.014.24991720
GAGCGGA851.6338642E-414.11797529
CCTTAAG2900.013.4540731
TTAGGTA4150.013.3730274
TATTAGG5000.013.2003072
ACAGTCC1601.2134478E-813.1245418
GAGTCGC1151.0940885E-513.0430238
AATACTA6900.012.6082555
CGCCGTT2451.8189894E-1212.24482625
CTAGACT1006.4537267E-411.999584
TATACTG6650.011.9544695
Produced by FastQC (version 0.11.2)