FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_18.35200000038a9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_18.35200000038a9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047789
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35150.17164854386853334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCAG3600.015.8312459
TAGGTAG4500.014.9983885
GGTAGTC4300.014.300797
CGGCGTG851.636261E-414.1157869
GAGCGAC1056.7631205E-512.85544811
ATCACAC4150.012.65264129
CTATGCA5000.012.5983399
CACCGCG1551.1338307E-712.58082923
GTATTAG6650.012.4116531
TTAGGTA6200.012.3376864
CCTAGCG1501.0385811E-612.00164130
TCACACA4750.012.0016430
TAGCCGG1006.458592E-411.9987127
GTATAGA5800.011.9019581
AGTCAGA5450.011.833302510
ATTAGGT6250.011.7590243
CTAATAC7200.011.6656993
GCTGCAC1551.5469486E-611.6116556
TATACCG3900.011.5372225
GTCAGAT5600.011.24851711
Produced by FastQC (version 0.11.2)