FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_17.35200000038a57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_17.35200000038a57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535258
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21500.14004160864167456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG4850.015.1587531
GGTAGTC2350.014.6800547
TCACACA4300.014.30434530
CTATGCA3700.014.1884189
GCCGCGT1106.907583E-613.637425
TAGTCAG2700.013.3326099
ATCACAC4850.013.30041429
CTAATAC5350.013.1768553
CTAGGAC800.001657526213.1242883
CGCCGCG1151.0933927E-513.0436224
ACTATGC4800.012.8118048
GCTAGCC954.1838567E-412.63336330
GTACTGC1551.1341399E-712.5799626
CCTAAGG1456.905193E-712.4131192
AGTCAGA3300.012.27166210
TAGGTAG3200.012.1868395
CCTAATA6700.012.0888952
CTTAGGC1006.4540876E-411.9993493
ACCTAAT6250.011.7631921
AGGCGCC1151.5798582E-411.7384947
Produced by FastQC (version 0.11.2)