FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_16.35200000038a06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_16.35200000038a06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708965
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28230.1651877013279968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG2850.016.8402825
GGTAGTC2950.014.7441677
TAGTCAG2850.014.2089889
CGCGCCT851.6344638E-414.11735722
TCCCGCG650.00639230913.84465512
GTATTAG5050.013.6682861
GGCTAGC800.001652496513.1297251
TTAGGTA4850.012.679044
GCGCGCC954.1899952E-412.6313221
CCTAGAG1456.9087946E-712.4128123
GGTCTGG850.002606113712.3573891
CTAGCCT1101.04452716E-412.2713994
CTATGCA4550.012.1964829
ACAGTGC1601.7166167E-712.1861818
ATCACAC3350.012.09213529
GTTAACC1252.5774423E-511.9990523
ATTAGGT5150.011.9408043
GTATTAA6350.011.5789711
CTAATAC6400.011.4834683
CGCGTTC1059.702412E-411.43034426
Produced by FastQC (version 0.11.2)