FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_15.352000000389de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_15.352000000389de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1736492
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25850.14886334057398481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG2500.015.5977845
CTAATAC6500.013.1519783
ACTATGC5450.012.9339438
ACTGTGC2450.012.8553168
GGTAGTC2950.012.2016567
TAGTCAG2600.012.1136649
ATCACAC3650.011.92057729
TTAGGTA5000.011.6983384
GCCGCAC1100.001435021910.9075418
GCTGTGC1100.001435021910.9075418
ATAGGGG1408.190502E-510.7127643
TCACACA4350.010.69213930
CTATGCC2402.6593625E-910.623499
GCCGTCT1000.00878186110.49971822
CCGTCGC1150.002078121510.43339
CTACGCT1150.002078121510.43334
GTCTTAA2453.7089194E-910.4111811
GTCTACA3051.2732926E-1110.3308631
TAATACT10350.010.2883934
TATACTG6900.010.21593955
Produced by FastQC (version 0.11.2)