FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_14.3520000003899b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_14.3520000003899b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584680
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25490.1608526642602923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG4200.012.14672851
CTTAGAC2351.546141E-1011.4883113
GGACTGC1059.7217876E-411.42752556
TTATACT5650.011.1494224
TGCGACG1355.6447287E-511.11219930
ATCGTGC950.0060023911.051628
CACCTCG950.0060023911.051627
ACACCGT2207.759809E-910.9080936
GGACAGC1100.001434296610.9080936
TATTAGG3850.010.90809252
TACACTG2751.8189894E-1110.90809255
ACAGTGC1951.898643E-710.7682468
TAGGTAG2651.09139364E-1010.7537335
GTATAGG3151.8189894E-1210.479531
CCTATAC2905.2750693E-1110.3438813
GTCTTAG2802.9831426E-1010.1818161
ACGGTGT2609.895302E-99.8067948
GGGTATA1705.3858545E-59.7089771
GGTGCGA1552.285127E-49.67836728
TAGGGAG1552.2894843E-49.6765355
Produced by FastQC (version 0.11.2)