FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n02_ntr_13.35200000038958.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n02_ntr_13.35200000038958.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1893545
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30770.16249943888315302No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG559.5883E-519.0896669
GTACTAG3750.013.605941
CTAGACT2051.9463187E-1012.4378864
CCTAGCG1202.3441775E-411.25075330
CGGGCGG950.006001943711.05191211
ACTAGAC2351.877197E-910.850073
GGTAGTC2104.573485E-810.7135887
GCGACGG1000.00878484710.4993157
GGAGCAC1304.920428E-410.3839398
TAGGGTG1756.6230623E-610.2847735
GTATAGA6300.010.2425671
ACGGTGT2505.2023097E-910.1993368
CGTACAC1653.899618E-59.9990853
TAATACT8850.09.9990844
CATACAC2609.902578E-99.8067943
TATACGG4150.09.7581425
CTAATAC5250.09.7133963
ACAGCGC1552.2894093E-49.6767898
ACCGTGT1552.2894093E-49.6767898
GTATTAG5600.09.6470691
Produced by FastQC (version 0.11.2)