FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_96.35100000039e2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_96.35100000039e2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694153
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT41630.5997236920390749No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC30950.44586712151355684No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC29600.42641895950892666No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC24560.3538124880249743No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG24080.3468975859788836No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT22750.3277375448928406No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC22210.3199582800909886No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG20770.29921357395271647No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT19050.2744351749542248No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC18440.2656474869373179No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT16380.23597103232284525No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG15800.227615525683819No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG15050.21681099123680228No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG15000.2160906889403345No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT14450.20816736367918887No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG14450.20816736367918887No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG13960.2011084011738046No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC13750.19808313152863996No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC13070.1882870202966781No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT12890.1856939320293941No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC12780.18410926697716498No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC12570.1810839973320003No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT12520.1803636950355325No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT11750.16927103966992868No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT11320.16307643992030577No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA11120.16019523073443462No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC10820.15587341695562792No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT10770.15515311465916015No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA10320.1486703939909501No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC10020.14434858021214345No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA9700.13973864551474963No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG9580.13800992000322695No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC9480.13656931541029138No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT9450.13613713403241073No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT9100.13109501795713624No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA9070.13066283657925556No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG9070.13066283657925556No Hit
GAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGT9040.13023065520137492No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT8960.12907817152702647No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT8960.12907817152702647No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC8910.1283578692305587No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT8770.1263410228004489No Hit
GTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTA8490.12230732994022932No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG8200.11812957662071619No Hit
CCGTTATCCGGATCGAGGACAGTGTATGGTGGGTAG8190.11798551616142262No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA8060.1161127301906064No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC7960.11467212559767082No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC7850.1130874605454417No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA7810.11251121870826748No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG7670.1104943722781577No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC7650.11020625135957059No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC7630.10991813044098346No Hit
GGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGA7550.10876564676663503No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT7530.1084775258480479No Hit
GCTAACTTTTCAAGGCCGAGAGCTGATGACGAGTGT7500.10804534447016725No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA7430.10703692125511234No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG7360.10602849804005744No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA7300.1051641352842961No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC7300.1051641352842961No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC7290.10502007482500256No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG7230.10415571206924122No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC7210.1038675911506541No Hit
GAGTTAGCCGGTGCTTATTCTGTCGGTAACGTCAAA6980.1005542005869023No Hit
GGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGG6950.10012201920902165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATA200.0051430330.0033930
ACTCGGT1600.025.3135343
CCCTAAA300.001225773425.001021
CTCGGTC1650.024.5482234
CCGTATG7250.023.585478
ACCGTAT7300.023.4239277
CGTATGC7350.023.264589
GTATGCG7500.022.7992910
TATGCGC4750.022.73449511
CCACCGT7750.022.6477175
CCCATAC402.4654611E-422.500921
TATGCGT2800.022.49767911
GTCCGAA553.880039E-621.8190732
CACCGTA8200.021.2219076
ATCCACC8150.021.1665083
TAGGCTC504.600366E-520.99934420
CATCCAC8250.020.9099432
ATGCGCA5300.020.65824512
CCCGTGC951.72804E-1020.5286334
TCCACCG8550.020.3531724
Produced by FastQC (version 0.11.2)