FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_95.35100000039de7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_95.35100000039de7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952870
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT77880.8173203060228572No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC48060.5043710054886815No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC39430.41380251240987753No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG33580.352409037959008No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG27860.2923798629403801No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT27330.2868177191012415No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC26970.28303965913503415No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT26640.2795764374993441No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC25840.27118074868555No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG25790.2706560181346878No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG24500.2571179699224448No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG24040.25229044885451324No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC23930.2511360416426165No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG23440.24599368224416762No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT22730.23854250842192537No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT21860.22941219683692426No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC20670.2169236097264055No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG20300.21304060365002572No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC18940.1987679326665757No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA18810.19740363323433419No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC18770.19698384879364444No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT18410.1932057888274371No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC18320.1922612738358853No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT17840.1872238605476088No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT16680.17505011176760732No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG16340.17148194402174483No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC15600.16371593186898528No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC15140.15888841080105365No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT14690.1541658358432945No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT14620.15343121307208749No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT14380.15091250642794926No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG14330.15038777587708713No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG13670.14346133260570698No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT13630.14304154816501727No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT13360.14020800319036175No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA12970.13611510489363712No Hit
GGTTCGCCTCTTGCACCTATGTATTCAGTACAAGAT12850.134855751571568No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT12850.134855751571568No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA12830.13464585935122314No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC12800.13433102102070588No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA12790.13422607491053345No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC12730.1335963982494989No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG12710.13338650602915403No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA12710.13338650602915403No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG12340.12950349995277424No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT12340.12950349995277424No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA12220.12824414663070513No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA12090.1268798471984636No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG12050.12646006275777388No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA11810.12394135611363566No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC11590.12163254168984225No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT11500.12068802669829043No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC11480.12047813447794557No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC11320.11879899671518675No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA11010.11554566729984153No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT10870.11407642175742758No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG10590.11113793067259961No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG10510.11029836179122021No Hit
GGCCTAGCCTTCTCCGTCCCTCCATCGCAATAACCA10450.10966868513018564No Hit
GGCTAGGCCAGCCTGGCGTTGGTTGTCCAGGTTTAA10410.10924890068949596No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC10380.10893406235897866No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT10260.10767470903690955No Hit
CGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACC10230.10735987070639226No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC10230.10735987070639226No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC10200.107045032375875No Hit
CTCCTCTACCGCATCATCAAAAGATGATACCCGTAG10140.10641535571484043No Hit
GAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGT10020.10515600239277131No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC9860.10347686463001249No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC9640.1011680502062191No Hit
CTTCTAAGCCAACCTCCTAGCTGTCTAAGCCTTCCC9550.10022353521466727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTC2200.025.9099084
GGTATAA300.001226175825.0007881
CCTCTGA1050.024.2839313
ACTCGGT2350.024.2560843
TACCTAG501.6943504E-624.00453627
TCTGACT1100.023.18011515
CTAGATA402.4639268E-422.50425130
CTCTTAT402.466554E-422.5007081
CTCTGAC1150.022.17228514
GTCTCTT553.877567E-621.82230630
TATTGGT350.003005231721.4292472
CCCAAAT350.003005231721.4292471
GAGCCAT5000.021.300671
CTTTCAT1500.021.0006624
GCCTCTG1450.020.68813512
TATGCGC5300.020.6588611
GTACCCG1400.020.3577841
CCACCGT9600.020.1568855
CCGTATG9650.020.050348
ACCTAGA608.23536E-620.00377828
Produced by FastQC (version 0.11.2)