FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_94.35100000039da4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_94.35100000039da4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679048
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT54470.8021524251599297No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC24990.3680152213098338No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC20180.2971807589448758No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG19910.2932046040927887No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG18260.26890587999670124No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG15240.2244318516511351No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT14760.21736313191409148No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG14480.2132397120674827No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT14470.21309244707296096No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG14370.21161979712774354No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT14090.20749637728113474No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC14080.207349112286613No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC13720.2020475724838303No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA13370.19689329767556935No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG12910.1901191079275692No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT12850.18923551796043872No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC12250.1803996182891342No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC11770.17333089855209058No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT11440.16847115373287308No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT11070.16302234893556863No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT10420.1534501242916554No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC10340.15227200433548144No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA9740.14343610466417692No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG9500.1399017447956551No Hit
GGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTT9230.13592558994356807No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC9190.13533652996548107No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG9070.13356935003122017No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC9020.13283302505861147No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT8980.1322439650805245No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT8930.13150764010791577No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG8880.13077131513530707No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT8790.1294459301846114No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC8740.12870960521200267No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT8580.12635336529965482No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA8500.12517524534348087No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT8390.12355533040374171No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG8240.12134635548591557No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA8140.11987370554069816No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC7890.1161920806776546No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC7860.11575028569408938No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC7780.11457216573791543No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA7750.1141303707543502No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT7650.11265772080913279No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG7500.11044874589130665No Hit
ATGTATCACTGTAGGGGGTAGAGCACTGTTTCGGCT7440.1095651559241762No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC7400.10897609594608924No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT7350.10823977097348052No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG7190.10588353106113266No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC7160.1054417360775674No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC7040.1036745561433065No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT6990.10293823117069781No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC6900.1016128462200021No Hit
CTTGTAGGTATTCGGAGTTTGCATCGGTTTGGTAAG6850.10087652124739341No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG6840.10072925625287167No Hit
CTTCTAAGCCAACCTCCTAGCTGTCTAAGCCTTCCC6820.10043472626382818No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA6810.10028746126930645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAC254.225136E-429.99926415
GTCTCTT359.966257E-525.71554830
ACTCGGT1100.023.1846663
ATAGAAC350.003002762621.4312023
GTCCATA350.003002762621.4312021
CTAGATA706.0954335E-821.42962330
CCTAGAT706.0954335E-821.42962329
AGTCTGT1550.021.2882338
ACAGTCT1500.021.0025776
CCCCCCT455.458877E-420.0024551
GTCTGTT1650.019.9980359
CATACCC9800.019.747323
CCTCTGA1003.43789E-1019.49808513
TATGCGC3550.019.43471111
GTGTAGG852.2115273E-819.4141481
CCGTATG5050.019.3050358
CCATACC10100.019.0122342
GCCATAC10150.018.918581
TACCCTT10000.018.902325
ACCGTAT5000.018.8981467
Produced by FastQC (version 0.11.2)