FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_93.35100000039d60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_93.35100000039d60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75718
Sequences flagged as poor quality0
Sequence length36
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT3470.45827940516125626No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCT2800.3697931799572096No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC2550.3367759317467445No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC2480.32753110224781423No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT2260.2984759238226049No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC2000.26413798568372115No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT1510.19942417919120947No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG1390.18357590005018623No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG1380.1822552101217676No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG1350.1782931403365118No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC1340.17697245040809317No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1300.17168969069441875No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT1260.16640693098074433No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC1210.15980348133865133No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT1210.15980348133865133No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC1180.15584141155339548No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1180.15584141155339548No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT1170.15452072162497688No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG1070.14131382234079082No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC1050.1386724424839536No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC1000.13206899284186058No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA970.12810692305660476No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTACG960.12678623312818615No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG930.12282416334293035No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC930.12282416334293035No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG900.11886209355767452No Hit
GGTACGCAGTCACCCAACAAAGTGGGCTCCCACTGC870.11490002377241872No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA870.11490002377241872No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT870.11490002377241872No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT840.11093795398716289No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG830.1096172640587443No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA820.10829657413032569No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC810.10697588420190708No Hit
ACCAGGAACCGTACCCCAAACCGACACAGGTGGTTG810.10697588420190708No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCTACGC790.10433450434506986No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC780.10301381441665126No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC780.10301381441665126No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT770.10169312448823264No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC760.10037243455981405No Hit
CTCCGAATACCTACAAGTGCCGAGCATGGGAGACAC760.10037243455981405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA200.00510406230.0158621
CCGAACT200.00510406230.0158624
ACCTCCT456.644932E-726.66314528
CAACCTC501.6649064E-623.9968326
AACCTCC501.6649064E-623.9968327
AAGCCAA501.6649064E-623.9968322
ACCGTAT452.0102572E-523.3302527
CCGTATG452.0102572E-523.3302528
CACCGTA402.4293727E-422.5118966
CATCCAC402.4293727E-422.5118962
CTCCTAG553.81075E-621.815330
TAAGCCA553.81075E-621.815321
TGCTTCT553.81075E-621.815315
ACCGTGT350.002982870221.4257418
GTATGCG504.5347493E-520.99722710
CCACCGT455.3828023E-420.0105745
GCTGCTT608.094636E-619.9973613
ACGAGAC608.094636E-619.9973622
TAAAGGG701.4023462E-619.2959124
GAGACGC701.4112757E-619.28316724
Produced by FastQC (version 0.11.2)