FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_92.35100000039d2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_92.35100000039d2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137684
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT8160.592661456668894No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC3770.27381540338746696No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC3700.2687312977542779No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT3430.24912117602626302No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG3200.23241625751721334No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG2950.21425873739868104No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC2910.2113535341797159No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC2860.2077220301560094No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG2820.20481682693704426No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT2690.19537491647540745No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG2260.16414398187153192No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT2230.16196507945730804No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG2080.15107056738618865No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC2080.15107056738618865No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC1950.14162865692455187No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC1910.1387234537055867No Hit
TCTCTACCCAACCACCTGTGTCGGTTTGGGGTACGG1880.13654455129136284No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG1820.1321867464629151No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC1780.12928154324394991No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA1770.12855524243920863No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1690.12274483600127829No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG1680.12201853519653699No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA1660.12056593358705442No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1630.11838703117283053No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT1600.11620812875860667No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG1600.11620812875860667No Hit
GGCCTAGCCTTCTCCGTCCCTCCATCGCAATAACCA1550.1125766247349002No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1540.11185032393015892No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC1530.11112402312541762No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG1520.11039772232067634No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC1490.10821881990645246No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC1470.10676621829696986No Hit
GTCCCAAAGTCATGGTTAAGTGGGAAACGATGTGGG1450.10531361668748729No Hit
GGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC1440.104587315882746No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG1390.10095581185903954No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1390.10095581185903954No Hit
TCTTTTGATGATGCGGTAGAGGAGCGTTCTGTAAGC1380.10022951105429825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAGG254.2072305E-430.06
GGCGTAG254.2072305E-430.011
GGAGGCG254.2072305E-430.08
CAATACA359.913106E-525.71428523
CGAGTCT300.001221076225.024
TAGCGAA300.001221076225.015
TCTTAAT300.001221076225.028
AGTCTTA300.001221076225.026
GTCTTAA300.001221076225.027
ATGCGCA900.024.99999812
ATCCACC1150.023.4782623
CGTATGC1200.022.59
TATGCGC1000.022.511
GCGCACA1000.022.514
TGCGCAC1000.022.513
ACCGTAT1200.022.57
CACCGTA1200.022.56
TGGCCTA402.4522367E-422.49999829
TGGCTTA755.151378E-922.021
CCGTATG1250.021.5999988
Produced by FastQC (version 0.11.2)