FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_91.35100000039ce8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_91.35100000039ce8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121237
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT10650.8784446992254841No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC4340.3579765253181785No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC4100.3381805884342239No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG3580.2952893918523223No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG3440.28374176200334883No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT3300.2721941321543753No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG3250.2680699786368848No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC2890.23837607331095292No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT2750.22682844346197945No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT2590.2136311522060097No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC2420.1996090302465419No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC2410.1987841995430438No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC2340.19301038461855705No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG2160.17816343195559112No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG2130.1756889398450968No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG2120.1748641091415987No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT2050.16909029421711194No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC2000.1649661406996214No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA1950.16084198718213089No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1910.15754266436813844No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1830.1509440187401536No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG1780.14681986522266305No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1720.14187088100167441No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG1710.1410460502981763No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC1680.13857155818768196No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC1640.13527223537368954No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC1560.1286735897457047No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT1540.12702392833870849No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG1490.12289977482121794No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1370.11300180637924066No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC1350.11135214497224445No Hit
GCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAA1350.11135214497224445No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA1330.10970248356524824No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC1310.10805282215825202No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT1310.10805282215825202No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA1310.10805282215825202No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA1310.10805282215825202No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC1300.1072279914547539No Hit
ACCATACACTGTCCTCGATCCGGATAACGGACCTGA1300.1072279914547539No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC1270.10475349934425959No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG1260.10392866864076149No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC1240.10227900723376528No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT1230.10145417653026716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTA554.62569E-927.27272813
GACTAGA505.5522833E-827.015
GTATTAC300.001220233225.03
TGTATTA300.001220233225.02
CTAGACG551.3974022E-724.54545617
ACTAGAC551.3974022E-724.54545616
TCTATTC702.3137545E-923.5714283
GTCTATT652.7348506E-823.0769232
TAGACGC603.2369826E-722.518
TATTACC402.4498877E-422.4999984
GCGGCTG402.4498877E-422.49999811
CTGTATT350.002990841421.4285721
CTCAACT350.002990841421.42857222
TGGCCTA350.002990841421.42857229
AGAGTTA350.002990841421.42857225
CACAGAG350.002990841421.42857222
ACAGAGT350.002990841421.42857223
TAAGGTG350.002990841421.4285725
TCTCAAC350.002990841421.42857221
GGTCTAT705.995025E-821.4285721
Produced by FastQC (version 0.11.2)