FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_90.35100000039ca5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_90.35100000039ca5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74330
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATG7320.9847975245526706No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCA3060.4116776537064442No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGGCAT2380.3201937306605677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT501.6545036E-624.0129281
TCTTATA904.0890882E-818.3432082
ATATACA500.001093027618.0096974
AGGCATG752.7290025E-617.99757830
TAGGCAT1151.542623E-411.7375529
ATATATA900.00393122111.6729522
CTTATAC1355.4543714E-511.1170973
TATATAT950.005883974511.0585861
TTATACA1304.7755626E-410.390214
ATACACA1602.6636832E-510.3180554
TATACAC1552.2099122E-49.6826323
TCCGAGC1409.49732E-49.6415613
TCTCCGA1450.00130923949.30909211
CACGAGA1500.00178349978.99878921
CTCCGAG1350.0076650528.88769212
TACACAT1550.00238804548.714375
ACTAGGC1550.00240271728.70850627
ACACATC1600.0031839258.4420456
GCCCACG1600.00320342238.43636418
CACATCT1600.00320342238.4363647
Produced by FastQC (version 0.11.2)