FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_89.35100000039c61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_89.35100000039c61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717520
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCGG1155.456968E-1219.56376510
GACTCCT1201.0913936E-1118.74991230
GAGCCCG1201.0913936E-1118.7486069
GCCCGGG1301.8189894E-1218.46016525
CCCGGGG1252.0008883E-1117.99866126
GGGCCCG1353.6379788E-1217.77645723
CCCGGCC1450.017.584912
GGAGCCC1303.45608E-1117.3064068
TGGGGGG1501.8189894E-1217.0046625
CGGCCCG1356.184564E-1116.66542814
CCCGGAG1356.184564E-1116.66542817
CCGGCCC1501.8189894E-1115.99881213
CGGAGGG1501.8189894E-1115.99881219
GGACTCC1351.1041266E-915.55548429
CCGGGGC1451.8189894E-1015.51608927
CTTAGGG600.00374525215.0041123
CCGGAGG1605.0931703E-1114.99888618
GCCCGGA1503.0377123E-1014.99888616
AGTGAGC600.003753946214.9988848
AAGGAGC2150.014.6551816
Produced by FastQC (version 0.11.2)