FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_88.35100000039c3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_88.35100000039c3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035077
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG851.6331526E-414.1176924
TCTAGAC1201.1219108E-613.7500443
AGGGGGG1753.0686351E-912.8571845
GAGAGCC850.002613789412.3517887
TGGGGGG1006.4478396E-412.0000385
AACGGCT900.00400942111.6655777
TAAGGGT1551.5424484E-611.6129414
TGTACTG1703.7594873E-711.4706255
CTTACAC1202.3436102E-411.2500363
CGAGCCC1602.2625572E-611.2489515
CTGTCCA1202.3457124E-411.2489499
CATATAC4700.011.1702493
ACTCCTG1755.4592056E-711.14450827
GCCTCAC950.00599577711.05266720
ACTGTCC1253.4236725E-410.7989918
GCACTGT1000.008775881510.5000336
ACAGCGC1000.0087821710.4990198
CCCTAAT1150.002074393210.4348171
GGACAGT1150.002074393210.4348176
CCTATAT2301.5465048E-810.4348171
Produced by FastQC (version 0.11.2)