FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_87.35100000039be9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_87.35100000039be9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236955
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCGA550.002102560416.3627110
TGGGGGG1401.8480932E-915.0021845
TAGGCCC851.6338276E-414.1174189
AGGGGGG2150.013.9555195
TAGGGGG1301.8259743E-713.848174
CTCCGAG1651.2751116E-913.635591512
AGTCCGG1004.2732936E-513.49923510
TTATACC2750.013.0928154
CATAGGC1302.7543683E-612.6941563
CTAAGAC1201.6909687E-512.501823
TGCGCGT850.002613564512.35224210
TTAGGGG1354.2016563E-612.2240013
CCGAGCC1857.024937E-912.16147314
GAGCCGG2102.8921932E-1012.14413429
GTCTTAG1151.5770411E-411.7403651
CCGGCCC1151.5794169E-411.73846613
TGACCCT900.00400302311.66836555
ATTATAC3600.011.66836553
CTACACA1459.316784E-611.3809674
ATACGAC1355.6396697E-511.1127283
Produced by FastQC (version 0.11.2)