FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_86.35100000039ba6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_86.35100000039ba6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113470
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGC703.1094816E-517.14465526
AGTCCGG908.588522E-716.66541910
GCCCGCC859.2092905E-615.88116416
TGGGGGG2150.015.3497575
GGCCCGC901.5825828E-514.99887815
CCCGCCC901.5825828E-514.99887817
CGCGGGG952.6323874E-514.21201525
CCCCCGC952.6357338E-514.21010121
CCGCGGG952.6357338E-514.21010124
CGGGGCG851.6319193E-414.11912627
TCCCGCG650.006389048413.84511812
GTCCGGC1106.9142698E-613.63534411
CCCGCGG1004.271473E-513.49959723
TCCGGCC1004.273276E-513.49898912
CCGGCCC1004.273276E-513.49898913
CTCCGAG1056.75413E-512.8561814
CGCCCCC1056.75413E-512.8561819
CCGAGCC954.1898803E-412.63063416
GGGCGGA850.002609241812.35479129
GCCCCCG1101.0431331E-412.2723620
Produced by FastQC (version 0.11.2)