FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_85.35100000039b62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_85.35100000039b62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460070
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCCG254.2240447E-429.99804320
CCCCCGC300.001225902624.9983721
GCCCGCC452.0382104E-523.33181216
GTAACTC400.00650714818.74877713
CGCCCCC400.00650714818.74877719
TGGGGGG1007.21775E-918.002745
CCGGCCC550.002099981816.36256813
CCCGCCC550.002099981816.36256817
TCCTACA653.4270392E-416.1563052
GTCCGGC653.4327208E-416.15279211
TCCGGCC755.6122444E-515.99895712
AGTCCGG706.0300936E-414.99902210
CGGCCCG706.0300936E-414.99902214
GGCCCGC706.0300936E-414.99902215
ACCTTGG809.737348E-514.9990227
TAGTCAG706.0300936E-414.9990229
CCGAGCC650.00638113313.84525114
CCTATAA800.001651885313.1269971
TAGTCCG800.001654592213.1241459
CTACACT850.002605179612.354824
Produced by FastQC (version 0.11.2)