FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_84.35100000039b2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_84.35100000039b2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751240
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG1002.5645222E-615.0034295
CCTAGCG600.00375325114.99943430
AGGGGGG1251.1277734E-714.4032925
CGCCGCG851.6318553E-414.11805524
TACGGAC750.001018171213.9985413
CACCTAG650.006384928713.84563228
TAGTCAG1004.2681626E-513.4994929
CCCGGCC902.65237E-413.331943518
GTACGGA902.65237E-413.331943512
GTCTATA800.001653459513.1271271
TCTCCGA1302.755938E-612.6918311
TATTAGG1907.6761353E-1012.6336252
GCCGCGT954.1834018E-412.63194325
CGGGAGC954.1834018E-412.63194326
CGAGACG954.1834018E-412.63194323
TCGCCGC850.002610242512.35329823
ACCTTGC850.002611546312.3524768
TGGTACG850.002611546312.35247610
CCCTTAG1006.435615E-412.0019441
TTGGGGG1006.435615E-412.0019444
Produced by FastQC (version 0.11.2)