FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_83.35100000039aea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_83.35100000039aea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875579
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCCG400.006506926418.7511775
TAGACGG500.00110946818.0011315
TTGGGGG859.192772E-615.8833524
CCTGTCC600.003754253314.99922858
TGGGGGG1201.1205902E-613.7508635
TAGGGGG1004.2657106E-513.5008484
AGGGGGG1352.8916293E-713.3341715
TCCGAGC1006.449049E-411.99938413
GACATAG1151.5758153E-411.74053930
GGATAGC900.004003899611.6673991
CCGAGCC1059.7109296E-411.42798414
GGTAGTC1459.328516E-611.3787257
CTCCGAG1202.3440129E-411.24942112
CCACGAG1202.3440129E-411.24942120
GTCTTAT1355.640059E-511.1118091
TAGTCAG1355.6466233E-511.1105399
TCCTAAG950.005991868711.0533272
ACCCTGC950.00599696511.0520648
TTACACT2053.192872E-810.9762994
CTTAAGG1100.001431340610.9097763
Produced by FastQC (version 0.11.2)